Other
Part:BBa_K1532000:Design
Designed by: Xinyuan Qiu Group: iGEM14_NUDT_CHINA (2014-09-29)
Cla I cutting site (methylation insensitive)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The original cutting site is sensitive to methylation, we re-engineered the sequence so that the methylation will not affect the cutting of Cla I.
Source
The coding of the Cla I cutting site is open-accessed.